文章摘要
初晓倩,初晓玲,秦军.[10]-姜酚对缺氧/复氧诱导的乳鼠心肌细胞损伤的保护作用及机制研究[J].药学与临床研究,2017,25(6):469~472
[10]-姜酚对缺氧/复氧诱导的乳鼠心肌细胞损伤的保护作用及机制研究
Protective effect of [10]-gingerol on hypoxia/reoxygenation induced neonatal rat cardiomyocyte injury and its mechanism
投稿时间:2017-07-25  修订日期:2017-12-15
DOI:
中文关键词: [10]-姜酚  缺氧/复氧  炎症
英文关键词: [10]-gingerol  hypoxia / reoxygenation  inflammation
基金项目:
作者单位E-mail
初晓倩 南京中医药大学附属八一医院 yichu311@163.com 
初晓玲 南京中医药大学附属八一医院  
秦军 南京中医药大学附属八一医院 qinjun312@163.com 
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中文摘要:
      目的:研究[10]-姜酚对缺氧/复氧诱导原代乳鼠心肌细胞损伤的保护作用及其机制。方法:提取原代乳大鼠心肌细胞,分为空白对照组、模型组、[10]-姜酚不同剂量组(1、3、10 μmol?L-1)。细胞缺氧4 h,复氧2h制备缺氧/复氧损伤模型;CCK-8法测定心肌细胞存活率;测定细胞上清液中生化指标,如乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB);测定上清液中炎症因子,如肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、白介素-1β(IL-1β)含量;Western blot 测定心肌细胞RhoA、ROCK1、p-NF-κB蛋白表达。结果:与模型组相比,[10]-姜酚增加乳鼠心肌细胞存活率,显著降低细胞上清液中LDH、CK-MB、TNF-α、IL-6、IL-1β含量。[10]-姜酚能降低心肌细胞中RhoA、ROCK1、p-NF-κB蛋白表达。结论:[10]-姜酚对乳鼠原代心肌细胞缺氧/复氧损伤具有保护作用,其机制可能与其抑制RhoA/ROCK1/NF-κB信号通路、减轻炎症反应相关。
英文摘要:
      Objective: To investigate the protective effects of [10]-gingerol on cardiomyocyte injury induced by hypoxia/reoxygenation in primary neonatal rats and its mechanism. Methods: Primary neonatal rat cardiomyocyte were divided into control group, model group, [10]-gingerol different dose group (1,3,10 μmol?L-1 ). Cells were treated with hypoxia for 4 h and reoxygenated for 2 h to induce hypoxia/reoxygenation injury. CCK-8 method was used to measure the viability of cardiomyocytes. Determination of biochemical markers in cell supernatants such as Lactate dehydrogenase (LDH) and Creatine kinase isoenzymes (CK-MB), Inflammatory factors such as Tumor necrosis factor-α(TNF-α), Interleukin-6 (IL-6) and Interleukin-1β (IL-1β). The expression of RhoA, ROCK1 and p-NF-κB in the cardiomyocytes were measured by Western blotting. Results: Compared with the model group, [10]-gingerol exhibited restored cell viability of hypoxia/reoxygenation induced neonatal rat cardiomyocytes and significantly decreased the contents of LDH, CK-MB, TNF-α, IL-6 and IL-1β in the supernatant.[10]-gingerol could reduce the RhoA, ROCK1 and p-NF-κB protein expression in cardiomyocytes. Conclusion: [10]-gingerol has protective effect on hypoxia/reoxygenation induced neonatal rat cardiomyocytes injury. The mechanism may be reduction of inflammatory response through inhibited of RhoA/ROCK1/NF-κB signaling pathway.
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