文章摘要
钱韵旭,赵浩如,高展.白花蛇舌草提取物的体外抗肿瘤活性[J].药学与临床研究,2004,12(4):36~38
白花蛇舌草提取物的体外抗肿瘤活性
The Anti-tumor Activity in vitro of Ethanol Extract from Oldenlandia diffusa
投稿时间:2004-05-31  
DOI:
中文关键词: 白花蛇舌草  抗肿瘤  体外  MTT法
英文关键词: Oldenlandia diffusa  Anti-tumor  In vitro  MTT assay
基金项目:
作者单位
钱韵旭 中国药科大学中药学院 
赵浩如 中国药科大学中药学院 
高展 中国药科大学中药学院 
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中文摘要:
      目的 探讨白花蛇舌草乙醇提取物(SCD)的体外抗肿瘤活性。方法 以MTT法测定SCD对体外培养的肿瘤细胞增殖的抑制作用、对人外周血单个核细胞的增殖促进作用及增强自然杀伤细胞杀伤肿瘤细胞的作用。结果 100μg/ml SCD对KB、BGC、B16、HBL-60、SMMC-7721、HELA、A549的增殖仰制率分别为30.53%、42.82%、23.77%、63.93%、39.40%、46.33%、42.56%;250μg/ml SCD可促进PBMC增殖97.33%;联合Con A或者LPS作用时,在0.25μg/ml的低浓度下增殖率分别达344.13%和152.18%;高浓度SCD未明显增强NK的杀伤作用。结论 SCD对体外培养的肿瘤细胞有明显的抑制作用,SCD可以显著促进PBMC增殖。
英文摘要:
      Aim To investigate the anti-tumor activity in vitro of ethanol extract from Oldenlandia diffusa(SCD).Method It was determined by MTT assay that the cytotoxic activity of SCD on cancer cell lines, the effect of SCD on the proliferation of PBMC and the anti-tumor activity of NK. Result the results showed that the inhibitory rate of SCD(100 μg/ml) on KB、BGC、B16、BL-60、SMMC-7721、HELA、A549 are 30.53%、42.82%、23.77%、63.93%、39.40%、46.33%amd 42.56% respectively. The proliferous rate of PBMC treated with 250 μg/ml of SCD was 97.33%. The rate of proliferation of PBMC activated by Con A or LPS incorporating with SCD (250 μg/ml) reached 344.13% and 52.18%. Low concentration of SCD could not increase the anti-tumor activity of NK. Conclustion SCD has a significance effect on restraining cultured cancer cell lines and enhancing the proliferation of PBMC.
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