文章摘要
刘晓玲,陈永刚,王犇.西格列汀对高脂饮食诱导的肥胖大鼠肾脏保护作用及相关机制研究[J].药学与临床研究,2018,26(4):245~248
西格列汀对高脂饮食诱导的肥胖大鼠肾脏保护作用及相关机制研究
Protective Effects and Related Mechanism of Sitagliptin on Kidney Injury Induced by High-fat Diet
投稿时间:2018-04-03  修订日期:2018-08-10
DOI:
中文关键词: 西格列汀  肥胖  肾脏损害
英文关键词: Sitagliptin  Fat  Kidney damage
基金项目:徐州科技局市课题项目资助(XZZDY1501)
作者单位E-mail
刘晓玲 徐州市中心医院临床药学部 lxlxz828@126.com 
陈永刚 徐州市中心医院临床药学部  
王犇 徐州市中心医院内分泌科 2064839318@qq.com 
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中文摘要:
      目的:观察西格列汀对肥胖大鼠肾脏是否具有保护作用并探讨其可能的机制。方法:雄性SD大鼠,随机分为正常对照组(NC)、肥胖组(OB)、西格列汀组(SIG)、阳性对照药厄贝沙坦组(IRB)。肥胖大鼠成模后,予以12周的用药干预,然后测定血糖(GLU)、血脂、氧化应激指标、尿微量白蛋白含量及肾皮质转化生长因子(TGF-β1)的表达情况。结果:用药干预后,与OB组大鼠相比,SIG组大鼠血脂、超敏C反应蛋白、尿微量白蛋白及尿肌酐明显降低,TGF-β1蛋白表达明显减弱,氧化应激水平明显增高,各指标间差异有统计学意义(P<0.01)。4组大鼠之间的GLU水平差异无统计学意义(P>0.05)。结论:西格列汀可降低肥胖大鼠尿微量白蛋白排出量,减轻肾小球结构的肥大,对肥胖大鼠的肾损伤具有一定的保护作用,其机制可能与影响TGF-β1信号通路、改善肥胖大鼠的脂质代谢紊乱、降低氧化应激及炎症反应有关。
英文摘要:
      Objective: To investigate the protective effects and the underlying mechanisms of sitagliptin treatment on kidney function in an obese rat model. Methods: Male Sprague-Dawley rats were randomly divided into three groups: normal control group (NC), obese group (OB), sitagliptin group (SIG) and Irbesartan positive control group (IRB). All obese rats were modeled and treated with drugs for 12 weeks. Their levels of blood glucose (GLU), blood lipids, oxidative stress indicators, urine microalbumin content, and renal cortical transforming growth factor (TGF-β1) expression were measured and analyzed. Result: The average values of blood lipid, high-sensitivity C-reactive protein, urine microalbumin, and urinary creatinine were significantly lower in the SIG group compared with the OB group after intervention with drugs. In contrast, we observed a significant reduction in the expression of TGF-β1 protein and an increase in the oxidative stress level. There were statistically significant differences among the indexes (P<0.01). There was no significant difference in GLU levels among the four groups (P>0.05). Conclusion: Sitagliptin can reduce urinary microalbumin excretion and glomerular hypertrophy in obese rats. It has protective effects on renal injury in obese rats. The mechanism may involve the inhibition of TGF-β1 signaling pathway. It can also improve lipid metabolism disorder and reduce oxidative stress and inflammatory reaction in obese rats.
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