文章摘要
宋樱花,胡浩彬.和血胶囊质量标准研究[J].药学与临床研究,2018,26(6):405~408
和血胶囊质量标准研究
Evaluation of the Quality of Hexue Capsules
投稿时间:2018-06-19  修订日期:2018-10-25
DOI:
中文关键词: 和血胶囊  薄层鉴别  高效液相色谱法  苍术素  5-羟甲基糠醛
英文关键词: Hexue capsule  TLC  HPLC  Atractydin  5-hydroxymethylfurfural
基金项目:
作者单位E-mail
宋樱花 南京中医药大学 276281541@QQ.com 
胡浩彬 江苏省食品药品监督检验研究院 2690170662@QQ.com 
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中文摘要:
      目的:修订并提高和血胶囊质量标准。方法:采用薄层色谱法对和血胶囊中苍术、皂矾进行鉴别;采用高效液相色谱法对苍术素与5-羟甲基糠醛进行含量测定。结果:薄层鉴别法斑点清晰,专属性强;5-羟甲基糠醛的测定可区分苍术是否经过炮制,5-羟甲基糠醛浓度在0.595 2~17.85 μg·mL-1范围内线性关系良好,r=0.999 9,平均回收率为102.6%,RSD为1.67%(n=6)。苍术素测定可有效控制苍术的投料量,苍术素浓度在1.076~32.29 μg·mL-1范围内线性关系良好,r=0.999 9;苍术素的平均回收率为101.7%,RSD为1.39%(n=6)。结论:所建立的方法操作简单、结果准确,可为和血胶囊的质量控制提供参考。 关键词 和血胶囊;显微鉴别;薄层鉴别;高效液相色谱法;苍术素;5-羟甲基糠醛
英文摘要:
      Objective: To revise and improve the quality standard of Hexue capsules. Methods: Copperas and atractylodis rhizoma in Hexue capsules were discriminated by microscopy and TLC. The contents of atractydin and 5-hydroxymethylfural were determined by HPLC. Results: The TLC method identified the crude drugs with clear spots and strong specificity. The determination of 5-hydroxymethylfural distinguished whether the atractylodes were processed or not. The concentration of 5-hydroxymethylfural was linearly determined in the range of 0.595 2-17.85 μg·mL-1, r=0.999 9, the average recovery rate was 102.6% and the RSD was 1.67% (n=6). The determination of atractydin effectively controlled the dosage of atractylodis rhizoma. The concentration of atractydin was linearly determined in the range of 1.076-32.29 μg·mL-1, r=0.999 9, the average recovery rate of atractydin was 101.7% and the RSD was 1.39% (n=6). Conclusion: The method is simple in operation and accurate in results, which can be referred for the quality control of Hexue capsules.
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